LIDo banner

Apply now

Find out more about the different routes to entry and our eligibility criteria

Bacteriophages as Generalised Synthetic DNA Delivery Vehicles

Bacteriophages as generalised synthetic DNA delivery vehicles

Bacteriophages are specialised nanomachines, delivering DNA payloads to specific types of bacteria via distinct interconnected parts. These parts are exchangeable: Any phage could be re-targeted to deliver DNA to any desired bacterial cell type. Although normally delivering instructions to copy itself, phages can be directed to deliver any genetic circuit to a bacterium. Artificial circuits could reprogram a cell’s repertoire with applications in microbial control, microbiome enhancement, or bioremediation. Following a previous LIDo DTP studentship, this project seeks to build on successes in phage nanomachine customisation, and extend novel payload concepts. We also seek to explore firewalling via cell-free approaches.

Disciplines and Techniques
Project supervisor/s
Dr. Renos Savva
Renos is interested in viruses of eukaryotes and prokaryotes.
Birkbeck University of London
Professor John M Ward
John is interested in microbial molecular biology,
University College London
Extending the Host Range of Bacteriophage Particles for DNA Transduction
Yosef, I., et al.
Mol Cell., 66(5): 721-728
Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing
Ando, H., et al.
Cell Systems, 1(3): 187-196
In Vitro Assembly of Infectious Virions of Double-Stranded DNA Phage Phi29 from Cloned Gene Products and Synthetic Nucleic Acids
Lee, C.S. and Guo, P.
J. Virol., 69(8), 5018-5023
Molecular Characterisation of Podoviral Bacteriophages Virulent for Clostridium perfringens and Their Comparison with Members of the Picovirinae
Volozhantsev, N.V., et al.
Plos One, 7(5): e38283
Multidisciplinary structural analysis of diverse picovirinae portal proteins enables applied particle engineering
Villanueva, H., Sattar, S., Javed, A., Vasciaveo, S., Pinheiro, V., Savva, R., & Orlova, E.
(manuscript in preparation)