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Kjetil Hansen: Improving Peptide Fragmentation for Hydrogen-Deuterium Exchange Mass Spectrometry Using a Time-Dependent Collision Energy Calculator

This publication analyses the effect of a mobility-dependant collision energy ramp on peptide ion fragmentation during hydrogen-deuterium exchange mass spectrometry. We showed that ramping collision energy as a function of peptide mobility, the coverage and peptide abundance was increased. Additionally we provided a streamlined python script to aid in calculation of improved collision energies.

Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is becoming a popular technique for interrogating biological systems. In recent years, advancements have been made to increase peptide coverage for proteins that resist digestion such as antibodies and membrane proteins. These methods commonly include using alternative digestion enzymes or longer chromatographic gradients, which may be expensive or time-consuming to implement.

Here, we recommend an efficient proteomics-based approach to increase peptide confidence and coverage. Click here to read full publication