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Next Generation Biomaterials Discovery Using Combinatorial Screening Technology

Next Generation

Biophysical parameters in the microenvironment, such as stiffness and topography, play a prominent role in determining cell proliferation and differentiation in vivo during health and disease. An example of this is epithelial to mesenchymal transition (EMT), an essential process during embryonic development and recapitulated during wound healing and tissue regeneration, but also significant in pathological settings such as cancer metastasis. A better understanding of EMT mechanisms and the influence of the microenvironment in this process hold significant value. Harnessing these mechanisms, through engineering the microenvironment in vitro, would provide a powerful tool for creating optimised cell substrates that could be utilised for enhancing cell bioprocessing, bioengineering tissue constructs and promoting tissue regeneration in vivo. However, identifying which physicochemical properties that achieve the target response is currently challenging. This project will use a fractional factorial design based approach to create combinatorial arrays of biomaterial-based cell substrates that will be used to screen reprogramming of epithelial cells into mesenchymal cells.

Disciplines and Techniques
Project supervisor/s
Professor Richard Day
Richard's research interests are centred on the application of biomedical engineering, primarily through tissue engineering and regenerative medicine, to provide healthcare solutions for debilitating conditions.
University College London
Dr. Caroline Pellet-Many
Caroline is interested in understanding the gene regulation as well as the molecular and cellular processes leading to cardiovascular diseases.
Royal Veterinary College
TIPS to manipulate myogenesis: retention of myoblast differentiation capacity using microsphere culture.
Parmar, N., Day, R.M
Eur Cell Mater. 30:41-50. doi:10.1155/2014/713631
Neuropilin 1 mediates epicardial activation and revascularization in the regenerating zebrafish heart
Lowe V, Wisniewski L, Sayers J, Evans I, Frankel P, Mercader N, Zachary C, Pellet-Many C
Development (in press)
Discuss the Epithelial to Mesenchymal Transition by the View of Mechanics
Ho-Kai HuangMing-Long Yeh
Using Acellular Bioactive Extracellular Matrix Scaffolds to Enhance Endogenous Cardiac Repair
Svystonyuk et al
Front. Cardiovasc. Med., 11 April 2018